The biomagnetic signature

Magnetic immunoassay was developed for the rapid and sensitive detection of proteins, viruses or bacteria in biological or food samples. The system incorporates the use of coated magnetic beads as labels in lateral flow (MIAstrip®) or flow through (MIAflo®) or chip (MIAchip®) formats.

In magnetic immunoassay, nano-sized magnetic beads are bound to a suitable antibody which specifically and selectively binds target molecules, micro-organisms or other antibodies in the test media.

 Solid phase coated with the primary antibody (1) Solid phase coated
with the primary antibody (Ab1)
  Specific immobilization of the analyte (2) Specific immobilization
of the analyte
 Specific labeling of the analyte (3) Specific labeling of the analyte by the Ab2/MB (Magnetic Bead) conjugate

Counting the number of MBs with a reader
(4) Measuring the magnetic response of MBs (magnetic beads)

The technology is versatile. It is compatible with multiple assays and uses standard liquid handling robots installed in many clinical labs worldwide.

The robots are designed to deliver reagents via removable tips to carry out ELISA on multi-well plates. As opposed to standard applications, the MIAflo® is realized on the 3D filters inside the tips, and the plate is used to hold the reagents. MIAflo® provides more sensitive and faster results compared to ELISA due to the absence of kinetic limitations and immunofiltration of the antigens on a large immune-active surface. In addition, MIAflo® successfully works even with the whole blood of patients, decreasing the sample preparation time.

The movie below demonstrates simultaneous detection and quantification of eight analytes in whole blood (no audio).